ANIEL KUMAR and SUBBA TATA (2010) In Vitro Shoot Bud Differentiation from Hypocotyl Explants of Chili Peppers (Capsicum annuum L.)

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In Vitro Shoot Bud Differentiation from Hypocotyl Explants of Chili Peppers (Capsicum annuum L.)

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CONTRIBUTORS:

	Author:	ANIEL KUMAR, Owk
	Author:	SUBBA TATA, Sape

JOURNAL:

Notulae Scientia Biologicae, 2(1), 66 - 69.

YEAR:

2010

PUB TYPE:

Journal Article

SUBJECT(S):

None

DISCIPLINE:

Plant Sciences

HTTP:

http://notulaebiologicae.ro/nsb/article/view/3553

LANGUAGE:

English

PUB ID:

103-470-244 (Last edited on 2010/03/24 03:46:04 GMT-6)

SPONSOR(S):

ABSTRACT:
Chili pepper (Capsicum annuum L.) is an economically important spice crop in tropical and subtropical countries. In vitro plant regeneration was obtained from 15th day old hypocotyl explants of three chili pepper cultivars (Capsicum annuum L., var. ‘X-235’, var. ‘PC-1’ and var. ‘Pusa Jwala’). Among the genotypes of Capsicum L. var. ‘X-235’ responded better than the var. ‘PC-1’ and var. ‘Pusa Jwala’. MS medium containing BAP (4.0 mg/l) and IAA (0.5 mg/l) was found to be the best medium for the production of maximum number of shoot buds in all the genotypes of chili pepper i.e., 6.80±0.16 (var. ‘X-235’), 5.00±0.19 (var. ‘PC-1’) and 4.80±0.12 (var. ‘Pusa Jwala’). The shoots were rooted on MS medium fortified with IBA (0.5 mg/l). Rooted plants were hardened and transplanted to the soil. The plants showed 80-90% survival during transplantation.

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