Sequence and Characterization of the Immunoglobulin Heavy Chain Constant and Partial Variable Region of the Mouse Strain 129/Sv
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CONTRIBUTORS:
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JOURNAL:
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Journal of Immunology,
179(4),
2419 -
2427.
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YEAR:
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2007
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PUB TYPE:
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Journal Article
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SUBJECT(S):
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None
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DISCIPLINE:
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No discipline assigned
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HTTP:
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LANGUAGE:
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None
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PUB ID:
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103-435-807
(Last edited on
2008/05/01 06:19:54 GMT-6)
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SPONSOR(S):
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ABSTRACT:
Although the entire mouse genome has been sequenced, there remain challenges concerning the elucidation of particular complex and polymorphic genomic loci. In the murine Igh locus, different haplotypes exist in different inbred mouse strains. For example, the Ighb haplotype sequence of the Mouse Genome Project strain C57BL/6 differs considerably from the Igha haplotype of BALB/c, which has been widely used in the analyses of antibody responses. We have sequenced and annotated the 3’ half of the Igha locus of 129/Sv, covering the CH region and approximately half of the VH region. This sequence comprises 128 VH genes, of which 49 are judged to be functional. The comparison of the Igha sequence with the homologous Ighb region from C57BL/6 revealed two major expansions in the germline repertoire of Igha. In addition, we found smaller haplotype specific differences like the duplication of 5 VH genes in the Igha locus. We generated a VH allele table by comparing the individual VH genes of both haplotypes. Surprisingly, the number and position of DH genes in the 129/Sv strain differs not only from the sequence of C57BL/6 but also from the map published for BALB/c. Taken together, the contiguous genomic sequence of the 3’ part of the Igha locus allows a detailed view of the recent evolution of this highly dynamic locus in the mouse.
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