Kube et al. (1995) Isolation of the human interleukin 10 promoter. Characterization of the promoter activity in Burkitt's lymphoma cell lines

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Isolation of the human interleukin 10 promoter. Characterization of the promoter activity in Burkitt's lymphoma cell lines

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CONTRIBUTORS:

	Author:	Kube, D
	Author:	Platzer, C
	Author:	von Knethen, A
	Author:	Straub, H
	Author:	Bohlen, H
	Author:	Hafner, Martin (Helmholtz Centre for Infection Research)
	Author:	Tesch H

JOURNAL:

Cytokine, 7(1), 1 - 7.

YEAR:

1995

PUB TYPE:

Journal Article

SUBJECT(S):

None

DISCIPLINE:

Medicine

HTTP:

http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WDF-45NJT71-M&_user=10&_coverDate=01%2F31%2F1995&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=ef11fa0620e3179a235f3f7f1f79f438

LANGUAGE:

English

PUB ID:

103-434-521 (Last edited on 2007/05/19 23:42:27 GMT-6)

SPONSOR(S):

ABSTRACT:
Interleukin 10 (IL-10) is a pleiotropic growth and differentiation factor with potent suppressor functions on macrophages, T cells and NK cells and contributes to the regulation of proliferation and differentiation of B cells. The expression of IL-10 appears to be tightly regulated, as the levels of constitutive expression in normal cells is extremely low. In contrast to normal haematopoetic cells, Epstein-Barr virus (EBV)-immortalized B cells and EBV-positive Burkitt's lymphoma cells express high levels of IL-10 constitutively. In this report we have cloned and sequenced IL-10 promoter fragments and analysed their activity in EBV-positive Burkitt's lymphoma cells. A nested set of DNA fragments from the IL-10 gene 5'-flanking region was placed upstream of the luciferase gene and assayed for their ability to direct luciferase expression in Burkitt's lymphoma cells. We have identified elements within the 5'-flanking region of the human IL-10 gene which can activate or suppress the constitutive expression of IL-10. The essential promoter of the IL-10 gene, which induces low levels of luciferase expression, was found to require the major start site of transcription (+1), a TATA-box (-77) and up to 150 additional 5' nucleotides. Positive regulatory sequences are located between -1100/-900. Negative regulatory elements which abolish luciferase activity were identified between -800/-300.

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